Anti-HA (YPYDVPDYA) FAST IP kit

Anti-HA (YPYDVPDYA) FAST IP kit

Background

Anti-HA-tag(YPYDVPDYA) fast IP kit is composed of Anti-HA affinity gel, Mouse IgG affinity gel, HA-Tag Rabbit pAb and Goat Anti-Rabbit IgG(peroxidase/HRP conjugated), which is used for fast, efficient and specific (Co)IP of HA tagged fusion protein.

Anti-HA affinity gel is made by covalently coupling high-quality HA-Tag Mouse mAb with agarose gel. It has the characteristics of high binding capacity of protein, high specificity and stability. Mouse IgG affinity gel is used as the control for CoIP experiment. The HA-Tag Rabbit pAb antibody has the advantages of high specificity, high affinity and high valence; Goat Anti-Rabbit IgG (peroxidase/HRP conjugated) is purified by cross-adsorption and only HA-Tag Rabbit pAb is identified, and there is no cross-reaction with the heavy-chain and light-chain of the mouse monoclonal antibody. The four components are strictly inspected and can be used independently; the combination of four sets has the advantages of fast, convenience and no interference band.

Performance

1.         Scope of application

(Co)IP of HA tag fusion proteins.

The HA tag can be located at the N-terminal, C-terminal or middle of Protein, such as N-terminal HA fusion protein (HA–Protein), C-terminal HA fusion protein (Protein-HA) and Met-modified N-terminal HA fusion protein (Met-HA–Protein).

2.         Antibody properties

HA-Tag Mouse mAb: Mouse IgG2a subtype;

HA-Tag Rabbit pAb: Rabbit IgG.

3.         Gel properties

Agarose gel granules, average size 50μm.

4.         Binding capacity

0.5mL Sepharose 4B agarose granules, covalently coupled with 4 mg of Anti-HA mouse monoclonal antibody.

Product Components

* Note: Buffer is PBS containing 50% glycerol.

Storage

-20°C, 12 months.