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Anti-Human CD8a-FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail– MSE Supplies LLC

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Elab Fluor® Violet 450 Anti-Human CD19 Antibody[HI19a]

Anti-Human CD8a-FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail

SKU: E-AB-FC0010-20

  • $ 13595
  • Save $ 1500

Anti-Human CD8a-FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail

 

SKU # E-AB-FC0010
Applications FCM

 

Product Details

Form Liquid
Clone No. OKT-8,RPA-T4,OKT-3,HI30
Reactivity Human
Application FCM
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Recommended Use For whole blood samples, add 5 μL Anti-Human CD8a-FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail to 100 μL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection. For other samples, 1×106 dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD8a-FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C and protected from prolonged exposure to light. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation FITC,PE,PE/Cyanine7,PerCP
FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g., a 525/40 nm bandpass filter). PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561 nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter). PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).

 

Fluorophore

Conjugation: FITC,PE,PE/Cyanine7,PerCP

FITC is designed to be excited by the Blue laser (488 nm) and detected using an optical filter centered near 530 nm (e.g.,a 525/40 nm bandpass filter). PE is designed to be excited by the Blue (488 nm), Green (532 nm) and Yellow-Green (561nm) lasers and detected using an optical filter centered near 575 nm (e.g., a 585/42 nm bandpass filter). PE/Cyanine7 is designed to be excited by the Blue (488 nm), Green (532 nm) and yellow-green (561 nm) lasers and detected using an optical filter centered near 775 nm (e.g., a 780/60 nm bandpass filter). PerCP is designed to be excited by the blue laser (488 nm) and detected using an optical filter centered near 675 nm (e.g., a 690/50 nm bandpass filter).

Recommended usage

For whole blood samples, add 5 μL Anti-Human CD8a-FITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail to 100 μL anticoagulant-treated blood sample. Mix and incubate the sample at 4°C in the dark for 30 min. Remove red blood cells with RBC lysis solution following the manufacturer's instruction. Wash the cell with cell staining buffer and discard the supernatant after centrifugation at 300 g for 5 min. Resuspend the cells with 200 μL cell staining buffer and load the sample on flow cytometer for detection.
For other samples, 1×106  dissociated single cells are centrifuged at 300 g for 5 min with the supernatant discarded. Resuspend the cells with 100 μL cell staining buffer and add 5 μL Anti-Human CD8aFITC/CD4-PE/CD3-PE/Cyanine7/CD45-PerCP Cocktail. Mix and incubate the sample at 4°C in the dark for 30 min. Add cell staining buffer to each tube, centrifuge at 300 g for 5 min and discard the supernatant. Resuspend the cells with
200 μL cell staining buffer and load the sample on flow cytometer for detection.