Anti-MYC (EQKLISEED) FAST IP kit

Anti-MYC (EQKLISEED) FAST IP kit

Background

Anti-MYC tag (EQKLISEEDL) fast IP kit is composed of Anti-MYC affinity gel, Mouse IgG affinity gel, MYC-Tag Rabbit mAb and Goat Anti-Rabbit IgG(peroxidase/HRP conjugated), which is used for fast, efficient and specific (Co)IP of MYC tagged fusion protein.

Anti-MYC affinity gel is made by covalently coupling high-quality MYC-Tag Mouse mAb with agarose gel. It has the characteristics of high binding capacity of protein, high specificity and stability. Mouse IgG affinity gel is used as the control for CoIP experiment, and its properties are stable. The MYC-Tag Rabbit mAb antibody has the advantages of high specificity, high affinity and high valence; Goat Anti-Rabbit IgG(peroxidase/HRP conjugated) is purified by cross-adsorption and only MYC-Tag Rabbit mAb is identified, and there is no cross-reaction with the heavy-chain and light-chain of the mouse monoclonal antibody. The four components are strictly inspected and can be used independently; the combined of four sets has the advantages of fast, convenience and no interference band.

Performance

1.         Scope of application

(Co)IP of MYC tag fusion proteins.

The MYC tag can be located at the N-terminal, C-terminal, or middle of the protein, such as the N-terminal MYC fusion protein (MYC–Protein), C-terminal MYC fusion protein (Protein-MYC) and the Met-modified N-terminal MYC fusion protein (Met-MYC–Protein).

2.       Antibody properties

MYC-Tag Mouse mAb: Mouse IgG2a subtype;

MYC-Tag Rabbit mAb: Rabbit IgG.

3.       Gel properties

Agarose gel granules, average size 50μm.

4.       Binding capacity   

0.5mL Sepharose 4B agarose granules, covalently coupled with 4 mg of Anti-MYC mouse monoclonal antibody.

Product Components

Note: Buffer is PBS containing 50% glycerol.

Storage

-20°C, 12 months.