ATTO AE-1435 EzApply2Dkit
SKU: AE-1435
ATTO AE-1435 EzApply2Dkit
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Purpose and Application:
- Extraction of Proteins from Mammalian cell or tissue cultures
- 2D electrophoresis sample buffer
Features:
- Separate extraction allows higher protein concentration per gel, spot detection is easy.
- Reducing reagent, alkylating reagent, washing buffer and stratified solutions are also incorporated into a kit
- Low running cost, while allowing short-time extraction and easy sample preparation
- Combination use with AE-1430 EzApply (sample preparation kit for SDS-PAGE) makes it applicable to SDS-PAGE as well as 2-D electrophoresis
MSE Supplies is an authorized distributor of ATTO products.
Technical Data:
| SKU# | AE-1435 |
| ATTO Model | AE-1435 |
| Major components |
Solution 1: Tris, Ampholine, DTT Solution 2: Urea, Thiourea, Surfactant Alkylated reagent: Ampholine, DTT Stratified solutions: Acrylamide, BPB, Urea |
| Package |
Solution/30 mL bottle x 2 (Wash buffer) Solution/20 mL bottle (Solution 1) Solution/10 mL bottle (Solution 2) Solution/1 mL tube (Alkylated reagent) Solution/500 μL tube (Stratified solution) Powder/tube (DTT) |
| Preparation | Appropriated amount of solution is applied to tissues or cells, which were lysed and extracted |
| Applicable amount | Extracted from 100 mg of mammalian tissue 20 runs |
| Storage |
6 months at -20℃ (before dissolved in DTT) 1 week after dissolved in DTT |