Bioneer AccuPower Dual-HotStart RT-PCR PreMix (480 T, 20 μl)

Bioneer AccuPower® Dual-HotStart™ RT-PCR PreMix (480 T, 20 μl)

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AccuPower^® Dual-HotStart™ RT-PCR PreMix is a one-step RT-PCR product that applied Pyro-Hotstart RT technology and Hotstart PCR technology to substantially improve the problems of non-specific reverse reaction. cDNA can be efficiently synthesized even with having small amounts of template RNA or being in a secondary structure. Thanks to its high sensitivity, accurate RT-PCR results can be obtained from various kinds of samples. Moreover, this product also contains all the components necessary for performing RT-PCR, allowing it to proceed just by adding the template RNA, primers and D.W.

※This product is shipped in dry ice.

Dual-HotStart™ Technology

1st Hotstart Reaction: Hostart RT

It uses a unique enzyme-mediated HotStart method that provides robust, sensitive, and reliable cDNA synthesis results. Bioneer's RocketScript™ reverse transcriptase is completely inhibited by pyrophosphate at temperatures below 50°C. However, RocketScript™ reverse transcriptase becomes fully active at temperatures above 50°C via pyrophosphate hydrolysis with a thermostable pyrophosphatases. This prevents the formation of mis-primed products and primer-dimers during the reaction set up process resulting in improved specificity of cDNA synthesis.

2nd Hotstart Reaction: HotStart PCR & qPCR

Bioneer's HotStart Taq Polymerase provides superior priming accuracy and specificity that cannot be achieved with other enzymes. You will use less enzyme per reaction, save money, and get higher sensitivity than with other HotStart enzymes.

Features and Benefits

Sensitivity

Detection of samples possible even at trace amounts of a target template in RNA with a high concentration that is generally difficult to detect

Comprehensive Applications

Excellent reactivity allowing gaining of accurate RT-PCR results even with template RNA samples of blood or soil samples containing various types of PCR inhibitors

Specificity

The world's first Dual Hotstart RT-PCR reaction using Pyro-Hotstart RT reaction and Hotstart PCR optimized to detect soley the target gene accurately.

Convenience

All components necessary for RT-PCR such as Thermostable DNA polymerase, RTase, dNTPs, etc all included, requiring only the addition of template RNA, primers and D.W.

Reproducibility

Mass produced in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product to provide reproducible experimental results

Applications

• Low copy viral/bacterial pathogen load determination in an earlier stage
• Low copy mRNA amplification
• Low copy target RNA quantification
• RNA amplification for microarray and NGS

Specifications