Bioneer AccuPower Dual-HotStart RT-qPCR Master Mix (2X, 2.5 ml)

Bioneer AccuPower® Dual-HotStart™ RT-qPCR Master Mix (2X, 2.5 ml)

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AccuPower® Dual-HotStart™ RT-qPCR PreMix is a one-step RT-PCR product that applied Pyro-Hotstart RT technology and Hotstart PCR technology to substantially improve the problems of non-specific reverse reaction and enhance the sensitivity to effectively undergo reverse transcription of template RNA even if it is present in a miniscule amount. Moreover, this product is compatible with comprehensive types of devices, including Exicycler™ 96 and those from other companies, by providing optimized tube and plates, along with a 2X master mix type..

※This product is shipped in dry ice.

Dual-HotStart™ Technology

1st Hotstart Reaction: Hostart RT

It uses a unique enzyme-mediated HotStart method that provides robust, sensitive, and reliable cDNA synthesis results. Bioneer's RocketScript™ reverse transcriptase is completely inhibited by pyrophosphate at temperatures below 50°C. However, RocketScript™ reverse transcriptase becomes fully active at temperatures above 50°C via pyrophosphate hydrolysis with a thermostable pyrophosphatases. This prevents the formation of mis-primed products and primer-dimers during the reaction set up process resulting in improved specificity of cDNA synthesis.

2nd hotstart reaction: HotStart PCR & qPCR

Bioneer's HotStart Taq Polymerase provides superior priming accuracy and specificity that cannot be achieved with other enzymes. You will use less enzyme per reaction, save money, and get higher sensitivity than with other HotStart enzymes.

Features and Benefits

Specificity

The world's first Dual-HotStart ™ RT-qPCR reaction using PyroHotStart RT reaction and HotStart PCR optimized to detect only the target gene precisely.

Sensitivity

Detection of miniscule amounts of the target templates in a high concentration of RNA samples unable to be done with conventional methods with a wide dynamic range of 10 logs up to 10 ~ 10¹⁰ copies.

Multiplex

Compatible with many kinds of fluorescent dye (probes) to detect various kinds of target genes.

Comprehensive Template RNA Detection 

RocketScript ™ RTase  included for undergoing RT reaction at high temperature and gaining RT-qPCR results from strong secondary template RNA structures.

Compatibility

Excellent reactivity towards template RNA allowing  accurate RT-qPCR results from wide choices of samples such as from blood and soils continaing various PCR inhibitors.

Convenience

All reactants necessary for r RocketScript ™ RTase, HotStart Taq DNA polymerase and cDNA synthesis and qPCR included in each tubes to readily start the one-step RT-qPCR by adding template DNA, probe & primer for target gene, and D.W.

Stability

Stabilizer included in the Real-Time PCR reaction mixture, being more stable the solution type products.

Reproducibility

Mass production under ISO 9001 quality system allowing minimized deviation between lots and reproducible results even for replicates tested repeatedly under the same conditions and variables.

Applications

• Gene expression profiling
• Target DNA quantification
• Microbial detection
• Viral/bacterial pathogen load determination

Specifications