Bioneer AccuPower PyroHotStart Taq PCR PreMix (96 T, 20 μl)

Bioneer AccuPower® PyroHotStart Taq PCR PreMix (96 T, 20 μl)

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AccuPower^® PyroHotStart Taq PCR PreMix is applied with Bioneer's enzyme-mediated HotStart patent technology to enhance response specificity and PCR amplification. Taq DNA polymerase reduces non-specific reactions like the mis-priming and primer dimer formation that may occur at a low temperature. In addition, the components necessary for PCR such as DNA polymerases, dNTPs, reaction buffers, etc. are thoroughly mixed and vacuum-dried, each packaged with amounts sufficient for a single PCR run.

※ This product is shipped at room temperature.

※ The reaction mixture contains stabilizers that keep full activity of enzymes even at the ambient temperature.

Features and Benefits

Specificity

Minimized generation of non-specific amplification products, maximized PCR reaction efficiency, and effective amplification of template DNA present in a small amount through the application of PyroHotStart technology.

Stability

Enhanced stability for maintaining stable enzymatic activities for a long-term storage by including stabilizer and dried in the PCR reaction mixture.

User-friendly

Simplified procedure with all necessary components and DNA polymerases added in the PCR tubes to perform the PCR reaction immediately just by adding the template DNA, primer sets, and D.W., along with tracking dye and sedimentation agents for electrophoresis without the need of sample loading buffer.

Reproducibility

Reproducible experimental results provided through mass production of one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product.

(a) Inhibition of PCR reaction in the zero cycle

After preparing the PCR mixture, PPi is used to prevent the formation of non-specific reactants before reaching the pre-denaturation temperature.

Also, since DNA polymerases requires Mg ions to activate, PPi, having high affinities with Mg2+ ions, traps them to inhibit the PCR.

(b) Denaturation

PPase included in the mixture is activated when the temperature rises during the denaturation step to decompose PPi into Pi for dissociating the Mg ions.

The isolated ions bind with DNA polymerases to activate, allowing the PCR reaction to proceed.

(c) Annealing & Extension

The activated DNA polymerases amplifies the target DNA as the PCR cycle progresses.

Applications

• High specificity PCR
• High sensitivity PCR
• gDNA template PCR
• Low-copy target PCR
• Multiple primer pairs PCR
• cDNA template PCR
• TA cloning

Specifications