Bioneer AccuPower RocketScript RT-PCR Master Mix (2X, 1ml)

Bioneer AccuPower® RocketScript™ RT-PCR Master Mix (2X, 1ml)

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AccuPower^® RocketScript™ RT-PCR Master Mix is the Master Mix type of AccuPower^® RocketScript™ RT-PCR Premix products.

※ Master Mix product is shipped in dry ice

Features and Benefits

Long kb RT-PCR

Synthesis of full-length size of 5.2 kb (target: human TFRC) target through one-step RT-PCR using human total RNA 100 ng.

Sensitivity

Synthesis using only 10 pg of human total RNA pg (target: ß-Actin) extracted from cells and tissues through one-step RT-PCR.

Thermostable Activity

Excellent thermal resistance of RocketScript ™ RTase active even at 70℃, making it suitable for cDNA synthesis of secondary RNA structure with comprehensive selection of compatible temperature ranging from 42 to 70 ℃ depending on the required experimental condition and effective cDNA synthesis.

Note: Schematic representation of the 5'UTR of a gene, with complex secondary structure, at three different temperatures. Note that RocketScript™ shows full activity at 70℃ allowing it to synthesize the complete gene sequence where M-MLV and other reverse transcriptase's fail.

Reproducibility

Mass produced in one-batch system under ISO 9001 quality system with thorough QC for each batch, then supplied as uniform quality product to provide reproducible experimental results.

User-friendly

All necessary components for cDNA synthesis such as thermostable reverse transcriptase, RNase inhibitor, etc., included to perform the reaction immediately just by adding the template RNA to be amplified, primer, and D.W. Tracking dye and sedimentation agents also included in the products for electrophoresis, making the loading buffer unnecessary to be added.

Applications

• First-strand synthesis of cDNA from RNA molecules (RT)
• RT-PCR
• cDNA library construction
• Gene expression analysis

Specifications