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Bioneer AccuRapid Protein Synthesis Kit (5 reactions)
SKU: K-7280
Bioneer AccuRapid™ Protein Synthesis Kit (5 reactions)
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AccuRapid™ Protein Synthesis Kit allows the expression and purification of desired proteins in vitro without culturing cells. This product contains all the components required for E.coli cell-free expression and Ni-affinity purification experiments, and can recover up to 100 μg of purified target protein per reaction.
※ This product is shipped in dry ice and ice pack by cartridge.
Features and Benefits
Convenience
Simple synthesis of protein without the need of cells, also allowing expression of proteins that can't be done by conventional cell culture methods.
User-friendly
Easy identification of the expression and purification of your desired protein from a variety of templates (plasmids or PCR products) that can be expressed in the T7 system.
Compatibility
Compatible with the world's first fully automatic protein synthesis equipment, ExiProgen™ EC Protein Synthesis Kit.
Specifications
Components
| Box No. | Components | Size | Storage Temperature |
| 1 | Ni-NTA magnetic bead | 50 mg x 5 ea | 4℃ |
| Elution buffer | 1.25 ml x 1 ea | ||
| Binding/washing buffer | 25 ml x 1 ea | ||
| 2 | *E. coli extract | 200 µl × 5 ea | -70℃ |
| Master mix | 350 µl × 5 ea | -20℃ | |
| Positive control DNA | 10 µl x 1 ea | ||
| DEPC D.W. | 1 ml × 1 ea |
*E. coli extract: As T7 RNA polymerase and ribosome are included, it is recommended to be stored at temperatures below -70 ℃ immediately upon the receive. Performance will decrease upon repeated freezing and thawing.