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Bioneer ExiProgen EC-Maxi Protein Synthesis Kit (8 reactions)
SKU: K-7310
Bioneer ExiProgen™ EC-Maxi Protein Synthesis Kit (8 reactions)
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ExiProgen™ EC-Maxi Protein Synthesis Kit allows automatic protein synthesis using our patented Stepwise Exchange Cell-Free (SECF) technology of ExiProgen™. After mounting the kit including the template DNA to the ExiProgen™, by operating the Protocol No. 903, up to 500 μg of proteins can be synthesized with a purity of 90% or more just in a single day. In addition, all reactions from protein expression/purification to dialysis are fully automated, with the final target proteins recovered dissolved in the desired storage buffer.
※ This product is shipped in dry ice and ice pack by cartridge.
Features and Benefits
Fully automated system
Automatic synthesis & purification of the target protein only within 25 hours just by activating ExiProgen™ mounted with the cartridge having the template DNA.
Concurrent multiprotein synthesis/purification/dialysis
Simultaneous synthesis, purification, and dialysis of 1 ~ 8 different kinds of proteins
Advanced protein synthesis system
Synthesis of complicated proteins difficult to be done in traditional in vivo expression systems such as cytotoxic proteins, membrane proteins, etc.
Reproducibility
Reproducible results through optimized protocols of automated devices to easily get about 500 μg protein.
Specifications
Components
| Components | Storage Temperature | ||
| Kit 1 | Cartridge ① | 96 well x 1ea | 4℃ Storage |
| Dialysis tube | 1 pack (16 ea/pack) | ||
| Disposable filter tip | 1 pack (8 ea/pack) | Storage at room temperature | |
| Protection cover | 1 ea | ||
| Kit 2 | Cartridge ② | 96 well x 1ea | -20℃ (E. coli extract recommended at -70℃ or less) |
| *E. coli extract | 8-strip tube (Yellow) x 1 ea | ||
| Master mixture | 8-strip tube (Violet) x 1 ea | ||
| DEPC D.W. | 8-strip tube (White) x 1 ea | ||
| Storage buffer | 35 ml x 2 bottle | ||
| Positive control DNA | 1.5 ml tube x 1 ea | ||
*E. coli extract (8 strip tube): As T7 RNA polymerase and ribosome are included, it is recommended to be stored at temperatures below -70 ℃ immediately upon the receive. Performance will decrease upon repeated freezing and thawing.