Bioneer ExiProgen EC Protein Synthesis Kit 96 (reactions)
SKU: K-7302
Bioneer ExiProgen™ EC Protein Synthesis Kit 96 (reactions)
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ExiProgen™ EC Protein Synthesis Kit allows protein synthesis through the use of ExiProgen™, installed with the world's first fully automated protein synthesis system. After mounting the kit containing template DNA on ExiProgen™ and activiating Protocol No. 902, within 6 hours, proteins are automatically expressed. The target proteins are then purified using His-tag. Maximum 16 different types of highly-pure proteins can be isolated simultaneously in a single reaction up to 100 μg. As the entire process is automated, unlike using the traditional manual procedures, having risks of getting different results depending on the experimental environment, this kit ensures reproducible results through each run.
※ This product is shipped in dry ice and ice pack by cartridge.
Features and Benefits
Fully automated system
Automatic synthesis & purification of the target protein only within 6 hours simply by inserting the template DNA into the cartridge and loading it in the ExiProgen ™
Advanced protein synthesis system
Synthesis of complicated proteins difficult to be done in traditional in vivo expression systems such as toxic proteins, membrane proteins, etc.
Diversity
Protein expression of wide size ranges from 10 kDa to 150 kDA from a variety of DNA including plasmid and DNA fragments from PCR products.
Reproducibility
Reproducible results through optimized protocols of automated devices.
Specifications
Components
Components | Storage temperature | |
Kit-1 (Purification kit) |
Cartridge ① | 4℃ |
Disposable filter tip | ||
Manual | ||
Kit-2 (Expression kit) |
Cartridge ② | -20℃ (E. coli extract recommended to be -70 ° C at most) |
Positive control DNA (1.5mL tube) | ||
*E. coli extract (8 strip tube) | ||
Elution tube and cap |
*E. coli extract (8 strip tube): It is recommended to store at temperature below -70℃ immediately upon recieve due to T7 RNA polymerase and ribosome. Repetitive freezing and thawing will reduce its performance.