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Bioneer MagListo His-tagged Protein Purification Kit (5 reactions)
SKU: K-7200
Bioneer MagListo™ His-tagged Protein Purification Kit (5 reactions)
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MagListo™ His-tagged Protein Purification Kit allows easy purification of His-tagged proteins through magnetic separation by Ni-NTA Magnetic Nanobeads and buffers. With an average diameter of 400 nm, the beads have large surface areas with excellent binding capacity and can be recovered quickly without any loss through their strong magnetic force. These characteristics can quickly reduce the experimental time and the increase the yield. About 3~4 mg of 6x His-tagged proteins can be bound on to about 1 ml of Magnetic Nanobeads.
※This product is shipped with ice pack.
Features and Benefits
Quick isolation
His-tagged proteins can be purified quickly and easily.
High-purity
Spherical shape magnetic beads with good binding ability allow high-purity isolation of target proteins.
Efficient Screening
Use of crude cell lysate for efficient screening.
Wide Application
Broad compatibility with all recombinant His-tagged protein purification with His-tag expressed in vivo (E. coli expression) or in vitro (cell-free expression).
Specifications
Components
| Components | Pack Size | Storage Temperature |
| Ni-NTA magnetic silica resin | 5 X 1 mL (10% slurry, v/v) | 4°C |
| Binding/Washing buffer | 100 mL | |
| Elution buffer | 15 mL | |
| Neodymium magnet | 3 ea | |
| User's Guide | 1 ea |
Procedure
Cell-shredded samples can be prepared in advance and processed according to the manual of this kit to purify 6x His-tagged protein.
※ Note
1. The neodymium magnet included in this kit can be used for purification.
2. This kit can be purified using centrifuge without neodymium magnet.
3. Bioneer's MagListo™-2(TM-1010), magnetic particle purification system, can make it easier and faster to purify 8 samples at a time.