Bioneer ProFi Taq DNA Polymerase, 10X Reaction Buffer without MgCl2, 20 mM MgCl2, 10 mM dNTPs (1,000 Units)

Bioneer ProFi Taq DNA Polymerase, 10X Reaction Buffer without MgCl2, 20 mM MgCl2, 10 mM dNTPs (1,000 Units)

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ProFi Taq DNA polymerase is improved from traditional Taq DNA polymerase with higher efficiency, making it suitable for long-range PCR. Furthermore, this enzyme is compatible with various target genes such as complex genomic DNA or cDNA templates, and low-copy targets.

※This product is shipped in dry ice.

Features and Benefits

Sensitivity

Higher amplification efficiency and sensitivity than other enzyme products by using lambda DNA and human gDNA as a template

Long Range PCR

Effective amplification especially lambda DNA up to 30kb and human gDNA up to 21kb.

Reproducibility

Reproducible results with uniform quality products for each batch under the ISO 9001 quality system

Applications

• Primer extension
• Long-range amplification from genomic DNA
• High amplification efficiency
• Excellent performance on difficult template
• Amplification of low-copy targets
• High yield and high sensitivity PCR.

Specifications

Component

10X reaction buffer with (or without) MgCl₂ : Tris-HCl, KCl, 15 mM MgCl₂, pH 9.0

Dilution buffer : 20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stabilizers, 50 % Glycerol, pH8.0

10 mM dNTPs mix: 2.5 mM of each dNTP

Concentration

250 Units (5 U/μl)

Storage Conditions

20 mM Tris-HCl, 0.5 mM EDTA, 1 mM DTT, 100 mM KCl, Stablizers, 50% Glycerol pH 8.0.

Storage Temperature

-20°C