Bioneer Top DNA Polymerase, 10 mM dNTPs, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)

Bioneer Top DNA Polymerase, 10 mM dNTPs, 10X Reaction Buffer without MgCl2, 20 mM MgCl2 (2,000 Units)

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Top DNA polymerase contains a thermostable DNA polymerase having improved polymerase activity by using the recombinant DNA technology on a thermophilic polymerase gene.

※This product is shipped in dry ice.

Features and Benefits

Optimized buffer delivery

Guaranteed stable response with Top optimized buffer for DNA polymerase

Efficiency & Sensitivity

High efficiency and sensitivity for DNA amplification using PCR 

Reproducibility

Reproducible results with uniform quality products for each batch by manufacturing under the ISO 9001 quality system.

Applications

• Real-time quantification of DNA and cDNA targets using dsDNA binding dye
• Gene expression profiling
• Microbial & viral pathogen detection.

Specifications

Components

10X reaction buffer with (or without) MgCl₂: Tris (pH 9.0), 15 mM MgCl₂, etc

1X dilution buffer: 50% glycerol containing 50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, pH 8.0

10 mM dNTPs mix: 2.5 mM of each dNTP

Concentration

500 Units (5 U/μl)

Storage Conditions

50% glycerol containing 50 mM Tris-HCl, 0.1 mM EDTA, 1 mM DTT, stabilizers, pH 8.0.

Storage Temperature

-20°C

Unit Definition

One unit is defined at the amount of enzyme that will incorporate 10 nmole of dNTP into acid-insoluble material in 30 minutes at 72°C.