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Ca2+-ATPase Activity Assay Kit
SKU: E-BC-K212-S-100
Ca2+-ATPase Activity Assay Kit
| SKU # | E-BC-K212-S |
| Detection Instrument | Spectrophotometer (660nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | Ca2+-ATPase |
| Sample Type | Animal tissue and cell |
| Sensitivity | 0.8 U/g wet weight |
| Detection Range | 0.8-41 U/g wet weight |
| Detection Method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 50 min |
| Precision | Average inter-assay CV: 8.800% | Average intra-assay CV: 4.100% |
| Other instruments required | Test tube, Micropipettor, Vortex mixer, Incubator, Centrifuge |
| Other reagents required | Normal saline (0.9% NaCl) |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| 10% Rat liver tissue homogenate | 5-8 |
| 10% Rat heart tissue homogenate | 5-8 |
| 10% Rat kidney tissue homogenate | 5-8 |
| 10% Mouse liver tissue homogenate | 1 |
| 10% Rat lung tissue homogenate | 5-8 |
| 10% Rat brain tissue homogenate | 2-3 |
Note: The diluent is normal saline (0.9% NaCl). For the dilution of other sample types, please do pretest to confirm the dilution factor.
Detection Principle
ATPase exists on the membrane of tissue cells and organelles. It is a kind of protease on the biological membrane which plays an important role in material transport, energy conversion and information transmission. Ca2+ which participates in the regulation of different enzyme systems and cell activities plays many important roles in cells. The flow of Ca2+ depends on the Ca2+ -ATPase on the cell membrane, and Ca2+ -ATPase consumes ATP to generate the energy needed for ion transport.
Kit Components & Storage
| Item | Component |
Size 1 (50 assays) |
Size 2 (100 assays) |
Storage |
| Reagent 1 | Buffer Solution | 10 mL x 1 vial | 20 mL x 1 vial | 2-8°C, 12 months |
| Reagent 2 | Activator A | 2 mL × 1 vial | 2 mL x 2 vial | 2-8°C, 12 months |
| Reagent 3 | Activator B | 2 mL × 1 vial | 2 mL x 2 vial | 2-8°C, 12 months |
| Reagent 4 | Substrate | Powder x 1 vial | Powder x 1 vial | 2-8°C, 12 months |
| Reagent 5 | Protein Precipitator | 10 mL × 1 vial | 10 mL × 1 vial | 2-8°C, 12 months |
| Reagent 6 | Chromogenic Agent A | Powder x 1 vial | Powder x 2 vials | 2-8°C, 12 months shading light |
| Reagent 7 | Acid Reagent | 25 mL x 1 vial | 50 mL × 1 vial | 2-8°C, 12 months |
| Reagent 8 | Chromogenic Agent B | Powder x 1 vial | Powder x 2 vials | 2-8°C, 12 months |
| Reagent 9 | 10 mmol/L Standard Solution | 2 mL x 1 vial | 2 mL x 1 vial | 2-8°C, 12 months |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three mouse kidney samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/kg wet weight) | 5.20 | 18.40 | 25.50 |
| %CV | 4.6 | 4.2 | 3.5 |
Inter-assay Precision
Three mouse kidney samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/kg wet weight) | 5.20 | 18.40 | 25.50 |
| %CV | 8.4 | 9.0 | 9.0 |
Sensitivity
The analytical sensitivity of the assay is 0.8 U/g wet weight. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.