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Canine E2(Estradiol) ELISA Kit
SKU: E-OSEL-C0001
Canine E2(Estradiol) ELISA Kit
| Detection Range | 15.63-1000 pg/mL |
| Sensitivity | 6.37 pg/mL |
Product Details
Properties
| Assay type | Competitive-ELISA |
| Format | 96T |
| Assay time | 1.5h |
| Detection range | 15.63-1000 pg/mL |
| Sensitivity | 6.37 pg/mL |
| Sample type &Sample volume | serum and plasma; 50μL |
| Specificity | This kit recognizes E2 in samples. No significant cross-reactivity or interference between E2 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Application | This ELISA kit applies to the in vitro quantitative determination of E2 concentrations in serum and plasma. |
Test Principle
This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Canine E2. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Canine E2 are added to the micro ELISA plate wells. Canine E2 in samples (or standards) competes with a fixed amount of E2 on the solid phase supporter for sites on the HRP linked detection antibody specific to E2. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Canine E2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Kit Components and Storage
An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
| Reference Standard | 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
| Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
| HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
| Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Technical Data:
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level E2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level E2 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (pg/mL) | 45.48 | 142.49 | 489.60 | 49.01 | 155.25 | 478.12 |
| Standard deviation | 2.62 | 6.28 | 15.08 | 3.32 | 7.27 | 22.14 |
| CV (%) | 5.77 | 4.41 | 3.08 | 6.78 | 4.68 | 4.63 |
Recovery
The recovery of E2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 90-103 | 96 |
| EDTA plasma(n=8) | 85-99 | 92 |
Target Information
| Synonyms | Oestradiol; E2; 17β-Estradiol; Estra-1,3,5(10)-triene-3,17β-diol |
| Research Area | Cell biology, Metabolism |
Assay Procedures
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1. Add 50µL each standard and sample. Immediately add 50µL of HRP linked Ab working solution. Incubate for 60 min at 37°C. |
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2. Aspirate and wash the plate for 5 times. |
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3. Add 90µL of Substrate Reagent. Incubate for about 15 min at 37°C. |
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4. Add 50µL of Stop Solution. |
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5. Read the plate at 450nm immediately. Calculation of the results |