Cell Invasion Assay | Collagen | 96 wells | Oris

3D Assay for Investigating Embedded Cell Movement through Type I Collagen

• Plate: 96-well plate with inserted Oris stoppers
  • Quantity: 1 plate, 96-wells per plate, treated with type I collagen
• Ancillary: Oris detection mask; Oris stopper removal tool
• Cellular matrix: Type I Collagen, 5 mg/mL
  • Quantity: 2 vials, 2 mL/vial
• Packaging: Plate is vaccum packaged inside metal-lined pouch with humidity absorber.  Collagen vials are packaged in ice.

Description

Oris : A SIMPLE PLATFORM FOR PUBLICATION-READY DATA

In the Oris 3D Cell Invasion Assay, stoppers are used to create a central collagen-containing Detection Zone for cell invasion experiments.

Cells are suspended in a thick layer of collagen surrounding a circular collagen Detection Zone.  After incubation, invading cells move into the detection zone, where they can be unambiguously quantified using a microscope or a plate-reader.

Features & Benefits

• Monitor cell invasion entirely in 3D
• Oris platform enables you to distinguish different cell behavior in 2D cell migration and 3D cell invasion assay.
• Physiologically Relevant: No artificial membranes or inserts required.
• Versatile: Supports real-time monitoring or end-point analysis
• Easy Analysis: Invasion can be quantified using image analysis or plate readers; no elaborate cell tracking software needed.