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Cholinesterase (CHE) Activity Assay Kit
SKU: E-BC-K052-M-96
Cholinesterase (CHE) Activity Assay Kit
| SKU # | E-BC-K052-M |
| Detection Instrument | Microplate reader (505-535 nm, optimum wavelength: 520 nm) |
| Detection Method | Colorimetric method |
Product Details
Properties
| Synonyms | CHE |
| Sample Type | Serum , plasma, animal tissue |
| Sensitivity | 2.17 U/mL |
| Detection Range | 2.17-71.33 U/mL |
| Detection Method | Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 60 min |
| Precision | Average inter-assay CV: 6.400% | Average intra-assay CV: 2.400% |
| Other instruments required | EP tube, centrifuge, Vortex mixer, 37℃ water bath, Microplate reader |
| Other reagents required | Normal saline (0.9% NaCl) |
| Storage | 2-8℃ |
| Valid period | 12 months |
Detection Principle
Cholinesterase breaks down acetylcholine into choline and acetic acid. Acetylcholine that is not hydrolyzed by cholinesterase reacts with hydroxylamine to form acetamidamine. It reacts in an acidic solution to form a brown-red hydroxamate iron complex. The color depth is directly proportional to the amount of remaining acetylcholine, which can be colorimetrically quantified. Cholinesterase activity was calculated.
Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 2(96 T) | Storage |
| Reagent 1 | Buffer Solution | 15 mL × 1 vial | 30 mL × 1 vial | 2-8℃, 12 months |
| Reagent 2 | Substrate | Powder × 1 vial | Powder × 2 vials | 2-8°C, 12 months, shading light |
| Reagent 3 | Diluent A | 1.5 mL ×1 vial | 1.5mL × 2 vials | 2-8°C, 12 months |
| Reagent 4 | Chromogenic Agent A | Powder × 1 vial | Powder × 1 vial | 2-8℃, 12 months |
| Reagent 5 | Alkaline Reagent | 10 mL ×1 vial | 20 mL × 1 vial | 2-8℃, 12 months |
| Reagent 6 | Acid Reagent | 12 mL × 1 vial | 24 mL × 1 vial | 2-8°C, 12 months |
| Reagent 7 | Protein Precipitator | 10 mL × 1 vial | 20 mL × 1 vial | 2-8°C, 12 months |
| Reagent 8 | Chromogenic Agent B | Powder × 1 vial | Powder × 1 vial | 2-8°C, 12 months, shading light |
| Reagent 9 | Diluent B | 0.5 mL × 1 vial | 1 mL × 1 vial | 2-8°C, 12 months |
| Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay(CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/mL) | 8.50 | 24.60 | 58.50 |
| %CV | 2.5 | 2.3 | 2.4 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/mL) | 8.50 | 24.60 | 58.50 |
| %CV | 5.9 | 6.8 | 6.5 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 102%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (U/mL) | 16.4 | 33.6 | 60.5 |
| Observed Conc. (U/mL) | 16.2 | 34.6 | 62.9 |
| Recovery rate (%) | 99 | 103 | 104 |
Sensitivity
The analytical sensitivity of the assay is 2.17 U/mL. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.