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Cyanine5.5 Labeling Kit (50 KD Filtration Tube)– MSE Supplies LLC

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Cyanine5.5 Labeling Kit (50 KD Filtration Tube) - MSE Supplies LLC

Cyanine5.5 Labeling Kit (50 KD Filtration Tube)

SKU: E-LK-C004C-10

  • $ 68095
  • Save $ 12000

Cyanine5.5 Labeling Kit (50 KD Filtration Tube)

 

Introduction

Elabscience® Cyanine 5.5 Labeling Kit provides all the reagents required for labeling, which can label proteins containing primary amino-group (-NH2) molecules simply and effectively.

 

Technical Information

Technical Information Elabscience® Cyanine 5.5 Labeling Kit provides all the reagents required for labeling, which can label antibodies and other proteins containing primary amino-group (-NH2) molecules simply and effectively.
Applications Protein labeling/Antibody labeling (50 KD Filtration tube)
Principle
Labeling group Cyanine 5.5
Spectrum
Storage 2~8°C/-20°C, shading light
Shipping Ice bag
Expiration date 12 months

 

Characteristic

  • Fast: The whole process takes only 90 min.
  • Convenient: The dye has been activated and can be used directly. Filtration tube desalts without dialysis.
  • Flexible use: It can be used for both micro-labeling and large-scale labeling, and can label 0.1-1 mg proteins each time.
  • Water solubility: Cyanine 5.5 in this kit is water-soluble, and the fluorescence characteristics of the dye can be well measured and maintained in aqueous buffer.

 

Essential Information

Excitation/Absorption maximum (nm)  675
Emission maximum (nm) 694
Molar extinction coefficient ε (L⋅mol−1⋅cm−1) 195000
Correction coefficient of 280nm(CF280) 0.11

 

Labeling Principle

Within a certain pH range, Cyanine 5.5 specifically reacts with primary amino groups (N-terminal and lysine residue side chains) to form a stable amide bond, so as to realize the coupling of Cyanine 5.5 with protein.

 

Materials Not Supplied

  1. Pipettor and tips (0.5-10μL, 2-20μL, 20-200μL, 200-1000μL).
  2. Ultraviolet spectrophotometer or nanodrop or multifunctional microplate reader.
  3. 37°C incubator.
  4. Centrifuge (centrifugal force up to 12,000×g).

Storage

The unopened kit can be stored at 2~8°C for 1 year, and the dissolved Cyanine 5.5 can be stored at -20°C or -80C for 1 week.

 

Notes
  1. Please select the appropriate kit according to the molecular weight of the protein to be labeled. The kit provides a 50 KD Filtration tube.
  2. Cyanine 5.5 is susceptible to moisture hydrolysis failure, and should be stored at -20°C or -80°C with the desiccant. In order to prevent water vapor from condensing into the dye, it is necessary to equilibrate the dye to room before the experiment.
  3. The kit can also be used to label other proteins containing free amino groups. The specific labeling ratio is determined according to the number of available amino groups in the marker or set different molar ratios for labeling.
  4. The optimal molecular ratio of the dye and protein (150KD) recommended for labeling with this kit is 18:1, which can ensure that the dye can label the protein, and DOS is in the normal range, but it cannot ensure the best experimental results. The optimal labeling ratio may vary according to the difference of protein, and the user can optimize according to the actual situation.