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D-Xylose Colorimetric Assay Kit
SKU: E-BC-K018-S-100
D-Xylose Colorimetric Assay Kit
| SKU # | E-BC-K018-S |
| Detection Instrument | Spectrophotometer(554 nm) |
| Detection Method | Colorimetric method |
Product Details
Properties
| Synonyms | D-aldopentose |
| Sample Type | Serum, plasma, urine |
| Sensitivity | 0.007 mmol/L |
| Detection Range | 0.007-4 mmol/L |
| Detection Method | Colorimetric method |
| Assay type |
Quantitative |
| Assay time | 30 min |
| Precision | Average inter-assay CV: 4.500% | Average intra-assay CV: 2.200% |
| Other instruments required | Micropipettor, Vortex mixer, Water bath |
| Other reagents required | Normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4) |
| Storage | 2-8℃ |
| Valid period | 12 months |
Detection Principle
D-xylose can produce furfural by dehydration in strong acid solution. The generated furfural reacts with Phloroglucinol to form pink compounds. The content of D-xylose can be calculated by colorimetric assay at 554 nm.
Kit Components & Storage
| Item | Component |
Size 1 (50 assays) |
Size 2 (100 assays) |
Storage |
| Reagent 1 | Phloroglucinol | 60 mL × 3 vials | 60 mL × 6 vials | 2-8℃, 12 months shading light |
| Reagent 2 | 13.3 mmol/L D-Xylose Standard | 1 mL × 1 vial | 1 mL × 1 vial | 2-8℃, 12 months |
| Reagent 3 | Standard Diluent | 10 mL × 1 vial | 10 mL × 1 vial 2 | 2-8℃, 12 months |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 0.05 | 1.20 | 3.50 |
| %CV | 2.5 | 2.0 | 2.1 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (mmol/L) | 0.05 | 1.20 | 3.50 |
| %CV | 4.3 | 4.8 | 4.4 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 103%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (mmol/L) | 0.1 | 1.5 | 3 |
| Observed Conc. (mmol/L) | 0.1 | 1.5 | 3.2 |
| Recovery rate (%) | 104 | 100 | 105 |
Sensitivity
The analytical sensitivity of the assay is 0.007 mmol/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.