DYKDDDDK-tagged Protein Purification Kit

DYKDDDDK-tagged Protein Purification Kit

Background

DYKDDDDK Tag is commonly used as eukaryotic protein recombinant expression marker. The DYKDDDDK tagged fusion protein purification kit is mainly composed of Anti-DYKDDDDK affinity agarose, which is made by covalent coupling of high-quality DYKDDDDK antibody and Sepharose 4B agarose granules. It has the characteristics of high binding capacity of protein, high specificity, stability, and repeatability. It can be used for affinity purification of DYKDDDDK tagged fusion proteins.

Performance

1.         Scope of application

Affinity purification of DYKDDDDK tag fusion protein.

DYKDDDDK tag can be located at the N-terminal, C-terminal or middle of the protein, such as N-terminal DYKDDDDK fusion protein (DYKDDDDK – Protein), C-terminal DYKDDDDK fusion protein (Protein DYKDDDDK) and Met modified N-terminal DYKDDDDK fusion Protein (Met DYKDDDDK – Protein).

2.         Gel properties

Agarose gel granules, average size 50 μm.

3.         Binding capacity

1mL Sepharose 4B agarose granules, covalently coupled with 8mg Anti-DYKDDDDK monoclonal antibody.

1mL affinity gel can purify at least 1.2mg DYKDDDDK fusion protein.

4.         Repeatability

It can be used repeatedly for more than 5 times.

5.         Components

1mL Anti-DYKDDDDK affinity gel, stored in 1mL PBS containing preservatives and 50% glycerol.

Product Components

Storage

-20°C, 12 months.