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Glucose Oxidase (GOD) Activity Assay Kit
SKU: E-BC-K520-M-96
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Glucose Oxidase (GOD) Activity Assay Kit
| SKU # | E-BC-K520-M |
| Detection Instrument | Microplate reader (540-560 nm) |
Product Details
Properties
| Synonyms | GOD |
| Sensitivity | 0.006 U/L |
| Detection range | 0.006–0.1 U/L |
| Assay type | Enzyme Activity |
| Assay time | 35 min |
| Precision | Average inter-assay CV: 7% | Average intra-assay CV: 3% |
| Other instruments required | Micropipette,Water bath, Centrifuge |
| Storage | -20℃ |
| Valid period | 12 months |
Detection Principle
Glucose oxidase can catalyze the oxidation of glucose to produce hydrogen peroxide. In the presence of peroxidase, hydrogen oxidizes pigment sources to form colored substances. Measure the OD value at 550 nm and glucose oxidase (GOD) activity can be calculated indirectly.
Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 1(96 T) | Storage |
| Reagent 1 | Buffer Solution | 15 mL × 1 vial | 30 mL × 1 vial | 20℃, 12 months |
| Reagent 2 | Enzyme Reagent | Powder × 1 vial | Powder × 2 vials | -20℃, 12 months shading light |
| Reagent 3 | Chromogenic Agent | 1.6 mL × 1 vial | 1.6 mL × 2 vials | -20℃, 12 months shading light |
| Reagent 4 | 1 mmol/L Standard | 1.6 mL × 1 vial | 1.6 mL × 2 vials | -20℃, 12 months shading light |
| Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay. (CV = Coefficient of Variation)
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/L) | 0.03 | 0.07 | 0.09 |
| %CV | 3.4 | 3.2 | 2.4 |
Inter-assay Precision
Three human serum samples were assayed 17 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/L) | 0.03 | 0.07 | 0.09 |
| %CV | 6.8 | 7.1 | 7.1 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 101%.
| Standard 1 | Standard 2 | Standard 3 | |
| Expected Conc. (mmol/L) | 0.28 | 0.54 | 0.83 |
| Observed Conc. (mmol/L) | 0.3 | 0.5 | 0.9 |
| Recovery rate (%) | 101 | 99 | 103 |
Sensitivity
The analytical sensitivity of the assay is 0.006 U/L activity. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.
Standard Curve
As the OD value of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique or temperature effects), so the standard curve and data are provided as below for reference only:
| Concentration (mmol/L) | 0 | 0.2 | 0.3 | 0.4 | 0.6 | 0.8 | 0.9 | 1 |
| OD value | 0.041 | 0.112 | 0.166 | 0.202 | 0.279 | 0.362 | 0.41 | 0.432 |
| 0.042 | 0.111 | 0.165 | 0.203 | 0.286 | 0.365 | 0.405 | 0.439 | |
| Average OD | 0.042 | 0.112 | 0.166 | 0.203 | 0.283 | 0.364 | 0.408 | 0.436 |
| Absoluted OD | 0 | 0.07 | 0.124 | 0.161 | 0.241 | 0.322 | 0.366 | 0.394 |