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Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)
SKU: E-BC-K143-100
Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)
| SKU # | E-BC-K143 |
| Detection Instrument | Biochemistry analyzer (340 nm), Spectrophotometer (340 nm) |
| Detection method | Colorimetric method |
Product Details
Properties
| Synonyms | Hcy |
| Sample Type | Serum |
| Detection Range | 0-50 μmol/L |
| Detection Method | Colorimetric method |
| Assay type | Quantitative |
| Storage | 2-8℃ |
| Valid period | 12 months |
Detection Principle
Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.
Kit Components & Storage
| Item | Component | Specification | Storage |
| Reagent 1 | S-adenosylmethionine | 37 mL × 2 vials | 2-8℃, 12 months, shading light |
| NADH | |||
| Tris (2-carboxyethyl) phosphonium chloride | |||
| α-ketoglutaric acid | |||
| Reagent 2 | HCY methyltransferase | 10 mL × 2 vials | 2-8℃, 12 months, shading light |
| Glutamate dehydrogenase | |||
| S-adenosine homocysteine hydrolase | |||
| Adenosine deaminase | |||
| Mannitol | |||
| Sodium azide | |||
| Reagent 3 | 0 μmol/L Homocysteine Standard | 1 mL × 1 vial | 2-8℃, 12 months |
| Reagent 4 | 28 μmol/L Homocysteine Standard | 1 mL × 1 vial | 2-8℃, 12 months |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.