Thank you!

Your quote has been successfully submitted!

For products requiring additional information, our team will contact you within 1 business day

Failed

There was an error submitting your quote. Please try again.

Lipase (LPS) Activity Assay Kit– MSE Supplies LLC

Free Shipping on MSE PRO Online Orders of $500 or More! U.S. Orders Only * Offer Excludes Hazmat Shipments *

Menu

Lipase (LPS) Activity Assay Kit

SKU: E-BC-K786-M-96

To better serve you, we would like to discuss your specific requirement.
Please Contact Us for a quote.

Lipase (LPS) Activity Assay Kit

SKU # E-BC-K786-M
Detection Instrument Microplate reader (440-420 nm, optimum wavelength: 412 nm)
Detection method: Colorimetric method


Product Details

Properties

Synonyms LPS
Sample type Serum, plasma, animal tissue, cell samples
Sensitivity 0.03 U/L
Detection range 0.03-9.41 U/L
Detection method Colorimetric method
Assay type Enzyme Activity
Assay time 70 min
Precision Average inter-assay CV: 6% | Average intra-assay CV: 3%
Other instruments required Incubator, Centrifuge, Microplate reader
Other reagents required Ethanol absolute (99.5%)
Storage 2-8℃
Valid period 12 months


Dilution of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
10% Mouse liver tissue homogenate 6-10
10% Rat brain tissue homogenate 2-6
10% Rat kidney tissue homogenate 3-6
10% Rat liver tissue homogenate 5-10
10% Rat heart tissue homogenate 2-5
10% Rat lung tissue homogenate 3-6
10% Rat spleen tissue homogenate 2-5
10% Mouse lung tissue homogenate 3-6
Human serum 7-10
Human plasma 2-6

 

Note: The diluent is buffer solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.

 

Detection Principle

Lipase can catalyze the substrate to produce sulfhydryl compounds, which react with DTNB to generate TNB. TNB has the maximum absorption at 412nm. The activity of LPS in the sample can be calculated by measuring the change of absorbance per unit time.

 

Kit Components & Storage

Item Component Size 1(48 T) Size 2(96 T) Storage
Reagent 1 Buffer Solution 30 mL × 1 vial 60 mL × 1 vial 2-8°C, 12 months
Reagent 2 Inhibitor 0.5 mL × 1 vial 1 mL × 1 vial 2-8°C, 12 months
Reagent 3 Substrate 3 mL × 1 vial 5 mL × 1 vial 2-8℃, 12 months
Reagent 4 Chromogenic Agent 0.9 mL × 1 vial 1.8 mL × 1 vial 2-8℃, 12 months, shading light
Microplate 96 wells No requirement
Plate Sealer 2 pieces

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.

 

Technical Data:

Parameter:

Intra-assay Precision

Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).

Parameters Sample 1 Sample 2 Sample 3
Mean (U/L) 0.85 2.40 6.80
%CV 3.3 2.9 2.8


Inter-assay Precision

Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.

Parameters Sample 1 Sample 2 Sample 3
Mean (U/L) 0.85 2.40 6.80
%CV 5.7 5.9 6.4


Recovery

Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 105%.


Sample 1 Sample 2 Sample 3
Expected Conc. (U/L) 1.7 4.6 8.5
Observed Conc. (U/L) 1.8 4.7 9.1
Recovery rate(%) 106 102 107

 

Sensitivity

The analytical sensitivity of the assay is 0.03 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.