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Lipase (LPS) Activity Assay Kit
SKU: E-BC-K786-M-96
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Lipase (LPS) Activity Assay Kit
| SKU # | E-BC-K786-M |
| Detection Instrument | Microplate reader (440-420 nm, optimum wavelength: 412 nm) |
| Detection method: | Colorimetric method |
Product Details
Properties
| Synonyms | LPS |
| Sample type | Serum, plasma, animal tissue, cell samples |
| Sensitivity | 0.03 U/L |
| Detection range | 0.03-9.41 U/L |
| Detection method |
Colorimetric method |
| Assay type | Enzyme Activity |
| Assay time | 70 min |
| Precision | Average inter-assay CV: 6% | Average intra-assay CV: 3% |
| Other instruments required | Incubator, Centrifuge, Microplate reader |
| Other reagents required | Ethanol absolute (99.5%) |
| Storage | 2-8℃ |
| Valid period | 12 months |
Dilution of Sample
The recommended dilution factor for different samples is as follows (for reference only):
| Sample type | Dilution factor |
| 10% Mouse liver tissue homogenate | 6-10 |
| 10% Rat brain tissue homogenate | 2-6 |
| 10% Rat kidney tissue homogenate | 3-6 |
| 10% Rat liver tissue homogenate | 5-10 |
| 10% Rat heart tissue homogenate | 2-5 |
| 10% Rat lung tissue homogenate | 3-6 |
| 10% Rat spleen tissue homogenate | 2-5 |
| 10% Mouse lung tissue homogenate | 3-6 |
| Human serum | 7-10 |
| Human plasma | 2-6 |
Note: The diluent is buffer solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.
Detection Principle
Lipase can catalyze the substrate to produce sulfhydryl compounds, which react with DTNB to generate TNB. TNB has the maximum absorption at 412nm. The activity of LPS in the sample can be calculated by measuring the change of absorbance per unit time.
Kit Components & Storage
| Item | Component | Size 1(48 T) | Size 2(96 T) | Storage |
| Reagent 1 | Buffer Solution | 30 mL × 1 vial | 60 mL × 1 vial | 2-8°C, 12 months |
| Reagent 2 | Inhibitor | 0.5 mL × 1 vial | 1 mL × 1 vial | 2-8°C, 12 months |
| Reagent 3 | Substrate | 3 mL × 1 vial | 5 mL × 1 vial | 2-8℃, 12 months |
| Reagent 4 | Chromogenic Agent | 0.9 mL × 1 vial | 1.8 mL × 1 vial | 2-8℃, 12 months, shading light |
| Microplate | 96 wells | No requirement | ||
| Plate Sealer | 2 pieces | |||
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.
Technical Data:
Parameter:
Intra-assay Precision
Three human serum samples were assayed in replicates of 20 to determine precision within an assay (CV = Coefficient of Variation).
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/L) | 0.85 | 2.40 | 6.80 |
| %CV | 3.3 | 2.9 | 2.8 |
Inter-assay Precision
Three human serum samples were assayed 20 times in duplicate by three operators to determine precision between assays.
| Parameters | Sample 1 | Sample 2 | Sample 3 |
| Mean (U/L) | 0.85 | 2.40 | 6.80 |
| %CV | 5.7 | 5.9 | 6.4 |
Recovery
Take three samples of high concentration, middle concentration and low concentration to test the samples of each concentration for 6 times parallelly to get the average recovery rate of 105%.
| Sample 1 | Sample 2 | Sample 3 | |
| Expected Conc. (U/L) | 1.7 | 4.6 | 8.5 |
| Observed Conc. (U/L) | 1.8 | 4.7 | 9.1 |
| Recovery rate(%) | 106 | 102 | 107 |
Sensitivity
The analytical sensitivity of the assay is 0.03 U/L. This was determined by adding two standard deviations to the mean O.D. obtained when the zero standard was assayed 20 times, and calculating the corresponding concentration.