Total Price: $0.00
Contact Information
Lipid Peroxide (LPO) Fluorometric Assay Kit
SKU: E-BC-F003-96
Lipid Peroxide (LPO) Fluorometric Assay Kit
| SKU # | E-BC-F003 |
| Detection Instrument | Fluorescence Microplate reader, Fluorescence Microscope, Flow Cytometry(Ex/Em=500 nm/525 nm) |
| Detection Method | Fluorescence method |
Product Details
Properties
| Synonyms | LPO |
| Sample type | Cell |
| Detection method | Fluorescence method |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid period | 12 months |
Detection Principle
Lipid peroxides (LPO) are substances produced by the action of reactive oxygen species (ROS) on unsaturated fatty acids. The LPO produced by the reaction will continue to oxidize the liposomes, thereby accumulating LPO in a chain reaction. Excess LPO decomposes into highly active aldehydes such as Acrolein, Malondialdehyde (MDA), and 4-hydroxy-2-nonenal (4-NHE), causing cell toxicity and cell death. This kit provides a fluorescent probe that can detect LPO production. This fluorescent probe can react with the lipid free radicals in the lipid peroxidation pathway, so as to detect the level of lipid peroxidation in cells. The probe fluoresces red under normal conditions through photoexcitation, and the fluorescence changes from red to green with the process of lipid peroxidation. Through the enhancement of green fluorescence intensity, the production of LPO can be detected with high sensitivity.
Kit Components & Storage
| Item | Component | Size (96 T) | Storage |
| Reagent 1 | Buffer | 50 mL ×2 vials | 20°C, 12 months |
| Reagent 2 | 5 mmol/L Probe | 0.05 mL × 1 vial | -20°C, 12 months shading light |
| Reagent 3 | 100 mmol/L Positive Control | 0.1 mL × 1 vial | -20°C, 12 months shading light |
| Black Microplate | 96 wells | No requirement | |
| Plate Sealer | 2 pieces |
Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.