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Long-arm Biotin Labeling Kit (50 kD Filtration Tube)– MSE Supplies LLC

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Long-arm Biotin Labeling Kit (50 kD Filtration Tube) - MSE Supplies LLC

Long-arm Biotin Labeling Kit (50 kD Filtration Tube)

SKU: E-LK-B004C-10

  • $ 85095
  • Save $ 15000

Long-arm Biotin Labeling Kit (50 kD Filtration Tube)

 

Introduction

Elabscience® Long-arm Biotin Labeling Kit provides all the reagents required for labeling, which can label proteins containing primary amino-group (-NH2) molecules simply and effectively.

 

Technical Information

Technical Information Elabscience® Long-arm Biotin Labeling Kit provides all the reagents required for labeling, which can label antibodies and other proteins containing primary amino-group (-NH2) molecules simply and effectively.
Applications Protein labeling/Antibody labeling (50 KD Filtration tube)
Principle
Labeling group NHS-LC-LC-Biotin

 

Characteristic

  • Fast: The whole process takes only 90 min.
  • Convenient: The NHS-LC-LC-Biotin has been activated and can be used directly. Filtration tube desalts without dialysis.
  • Flexible use: It can be used for both micro-labeling and large-scale labeling, and can label 0.1-1 mg proteins each time.
  • Long arm fat-soluble: NHS-LC-LC-Biotin in this kit is fat-soluble long arm, which increases the spatial distance between biotin group and protein, reduces the spatial binding hindrance, and may be more effective for the binding of streptavidin.

 

Materials Not Supplied

1. Pipettor and tips (0.5-10μL, 2-20μL, 20-200μL, 200-1000μL).
2. 37°C incubator.
3. Centrifuge (centrifugal force up to 12,000×g).

 

Shipping & Storage Information

Storage 2~8°C/-20°C, shading light
Shipping Ice bag
Expiration date 12 months

Notes
  1. Please select the appropriate kit according to the molecular weight of the protein to be labeled. The kit provides a 50 KD Filtration tube.
  2. NHS-LC-LC-Biotin is susceptible to moisture hydrolysis failure, and should be stored at -20°C or -80°C with the desiccant. In order to prevent water vapor from condensing into the NHS-LC-LC-Biotin, it is necessary to equilibrate the NHS-LC-LC-Biotin to room before the experiment.
  3. The kit can also be used to label other proteins containing free amino groups. The specific labeling ratio is determined according to the number of available amino groups in the marker or set different molar ratios for labeling.
  4. The optimal molecular ratio of the NHS-Biotin and protein (150KD) recommended for labeling with this kit is 20:1, which can ensure that the NHS-Biotin can label the protein, but it cannot ensure the best experimental results. The optimal labeling ratio may vary according to the difference of protein, and the user can optimize according to the actual situation.