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Mitochondrial Extraction Assay Kit– MSE Supplies LLC

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Mitochondrial Extraction Assay Kit - MSE Supplies LLC

Mitochondrial Extraction Assay Kit

SKU: E-BC-E001-100

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Mitochondrial Extraction Assay Kit

SKU # E-BC-E001


Product Details

Properties

Sample type Animal tissue
Other instruments required High-speed freezing centrifuge, 5mL Glass homogenizer
Other reagents required Double distilled water, PBS(0.01 M,pH=7.4)
Storage -20℃
Valid period 12 months

Detection Principle

The principle of mitochondrial separation and purification: after rupturing cells mechanically, the debris and huge organelles are removed by low-speed differential centrifugation, then purified mitochondria will be obtained by high-speed differential centrifugation.

 

Kit Components & Storage

Item Component Specification Storage
Reagent 1 Lysis Buffer 60 mL × 1 vial -20°C, 12 months
Reagent 2 Mito-Wash Buffer 30 mL × 1 vial  -20°C, 12 months, shading light
Reagent 3 Store Buffer 15 mL × 1 vial  -20°C, 12 months

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.