MSE PRO Cell Isolation Optimizing System

MSE PRO™ Cell Isolation Optimizing System

The MSE PRO™ Cell Isolation Optimizing System a complete method development kit containing the most commonly used enzymes for enzymatic tissue dissociation and cell separation procedures. Includes instructions, references, and strategies for handling, using, and optimizing enzymatic cell separation methods for maximum live cell yield. Contains all enzymes commonly used in tissue dissociation and cell isolation procedures, as well as a Guide to Cell Isolation, which describes commonly used tissue types, modes of action of various enzymes, tissue culture techniques, and protocol optimization guidelines, with cell- and tissue-specific references to get started with enzymatic cell isolation.

Although enzymes have been widely used in these applications for many years, the mechanisms of enzyme action in dissociation and harvesting are not well understood. As a result, the choice of one technique over another is often arbitrary and based more on past experience than on an understanding of why the method works and what modifications might lead to better results. Much of this difference stems from the complexity and dynamics of the extracellular matrix and the relatively crude, undefined enzyme preparations that have been used in cell separation applications in the past. The extracellular matrix consists of a wide variety of proteins, glycoproteins, lipids, and glycolipids, all of which can vary in abundance depending on species, tissue, and developmental age. Our Cell Separation Optimization System provides a wide variety of widely used enzymes in purified form for establishing cost-effective and optimized cell separation procedures.

Brand: MSE PRO™