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MSE PRO Hydroxysteroid Dehydrogenase
SKU: BO0270
MSE PRO™ Hydroxysteroid Dehydrogenase
The MSE PRO™ Hydroxysteroid dehydrogenase catalyzes the interconversion of the hydroxyl and carboxyl groups of steroids.There are two types of hydroxysteroid dehydrogenases derived from P. testosteroni: 3-alpha-hydroxysteroid dehydrogenase (α-enzyme) and 3-beta-hydroxysteroid dehydrogenase (β-enzyme).The molecular weight of the α-enzyme is 47,000 daltons.The α-enzyme oxidizes only the C19, C21, and C24 series of 3-alpha- hydroxysteroids. It is inhibited by heavy metals and sulfhydryl-binding agents. β-enzyme catalyzes the oxidation of 3-β-hydroxysteroids of the C19 and C21 series, 17-β-hydroxysteroids of the C18, C19, and C11 series, and some 16-β-hydroxysteroids. It is inhibited by heavy metals and reducing agents. Oxidation of testosterone is inhibited by 3,17-alpha-estradiol and other 1,3,5-estradiol derivatives. We offer two preparations: one from a conventional ferritin (ATCC 11966) culture that produces both α and β enzymes, and the other from a mutant strain that produces almost exclusively α enzymes. By using both, beta-hydroxysteroids can be identified by differences.
Brand: MSE PRO™
Manufacturer: MSE Supplies LLC
MSE Supplies offers a variety of Biochemical Reagents. Please contact us for SDS, lead time and more information.
Applications:
Preparation of a composite electrode platform for the determination of androstenedione; in potassium phosphate buffer for the determination of bioluminescence of bile acids in serum; and for the determination of total bile salts by enzymatic methods.
Specifications:
| SKU# | BO0270 |
| EC No. | 1.1.1.51 |
| Biological source | Pseudomonas testosterone |
| Activity | ≥0.5 U/mg dw |
| Storage | -20°C |
| Unit Definition | One unit reduces 1 μmol of NAD per minute at 25°C, pH 9.0 with androstenedione or testosterone as substrate. |
| Note | Product packaging is subject to change, the picture is for reference only |