MSE PRO Pepsin A
SKU: BO0159
MSE PRO™ Pepsin A
The MSE PRO™ Pepsin is a major protease in the human and porcine stomach, a small gastric protein hydrolyzing enzyme similar to pepsin, secreted by the gastric mucosal host cells in the stomach, which functions to break down food proteins into small peptide fragments. It contributes to the protein hydrolysis of food proteins in the stomach of vertebrates. Pepsin is a monomer with two structural domains, mainly B proteins with a high proportion of acidic residues. Porcine pepsin has 4 basic and 42 acidic residues. Pepsin is the major protein hydrolase produced in the stomach. It digests proteins by cleavage of internal peptide bonds. Pepsin is highly active in acidic environments, with an optimal pH of about 3. In neutral or alkaline pH solutions, above pH 7 pepsin undergoes irreversible denaturation, whereby pepsin undergoes unchaining and loses activity. Pepsin activity can be inhibited by Pepstatin. It is hygroscopic; aqueous solutions show an acidic reaction.
Brand: MSE PRO™
Manufacturer: MSE Supplies LLC
MSE Supplies offers a variety of Biochemical Reagents. Please contact us for SDS, lead time and more information.
Applications:
Can be used for protein hydrolysis, can decompose the peptide bond formed by aromatic amino acids or acidic amino acids in proteins, can hydrolyze globular proteins, histones and catalytic plant proteins. It can also be used as antibody digestion. In the cosmetic industry, it can be used for the preparation of collagen; it can also be used to prevent the clouding of beer in cold storage; it can also be used for the evaluation of protein digestibility in food chemistry.
Specifications:
| SKU# | BO0159 |
| CAS No. | 9001-75-6 |
| EC No. | 3.4.23.1 |
| EINECS No. | 232-629-3 |
| Biological Source | Pig stomach |
| Activity | ≥2,500 U/mg dw |
| Molecular weight | 35 kDa |
| Unit Definition | At 37°C, one unit releases 0.001 A280 per minute from denatured hemoglobin as a soluble hydrolysis product of TCA. One FIP unit is expressed as μmol tyrosine equivalents released per minute at 25°C and is calculated as follows: 1 our unit x 0.0071 = FIP unit. |
| Storage | 2-8°C |
| Note | Product packaging is subject to change, the picture is for reference only |