One-step TUNEL Flow Cytometry Apoptosis Kit (Blue, Elab Fluor® Violet 450)

One-step TUNEL Flow Cytometry Apoptosis Kit (Blue, Elab Fluor® Violet 450)

Introduction

Elabscience® One-step TUNEL Flow Cytometry Apoptosis Kit applies a highly sensitive, fast and simple method to detect cell apoptosis. This kit can be used to detect apoptosis of suspension cells and adherent cells by flow cytometry.

Product Details

Properties

Detection Principle

When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. The DNA of apoptotic cells is cleaved into multimers of 180~200bp fragments, corresponding to the oligonucleosomal size. Therefore, the DNA of apoptotic cells typically migrates as a ladder of 180~200bp on an agarose gel. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with fluorescein labeled dUTP, which can be detected with flow cytometry.

Storage

The kit can be stored at -20°C for 12 months. Labeling Solution should be stored in the dark. It is recommended to aliquot the Fixation Buffer and Permeabilization Buffer into smaller quantities and avoid freezing and thawing cycles.

Notes

1. This kit is for research use only.
2. Please take safety precautions and follow the procedures of laboratory reagent operation.
3. The minimum number of cells for this kit should not be less than 5×105.
4. When resuspending the cells, gently pipette the cells 10~20 times. Avoid blowing out the liquid in the pipette completely each time to avoid cell damage and excessive bubbles.
5. Be careful to remove the supernatant after centrifugation to avoid the loss of cells.
6. Don’t vortex the Labeling Solution and TdT enzyme and avoid freezing and thawing cycles.