QuicKey Human TG(Thyroglobulin) ELISA Kit

SKU: E-TSEL-H0025

$ 331⁹⁵
Save $ 59⁰⁰

QuicKey Human TG(Thyroglobulin) ELISA Kit

Detection Range	15.63-1000 pg/mL
Sensitivity	3.38 pg/mL

Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T
Assay time	2.5h
Detection range	15.63-1000 pg/mL
Sensitivity	3.38 pg/mL
Sample type &Sample volume	serum and plasma; 50μL
Specificity	This kit recognizes Human TG in samples. No significant or interference between Human TG and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Human TG concentrations in serum and plasma.Please consult technical support for the applicability if other biological fluids need to be tested.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human TG. Samples (or Standards) and biotinylated detection antibody specific for Human TG are added to the micro ELISA plate wells. Human TG would combine with the specific antibody. Then Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human TG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 ± 2 nm. The OD value is proportional to the concentration of Human TG. You can calculate the concentration of Human TG in the samples by comparing the OD of the samples to the standard curve.

Kit Components and Storage

An unopened kit can be stored at 2-8℃ for six months. After test, the unused wells and reagents should be stored according to the table below.

Item	Specifications	Storage conditions after test
Micro ELISA Plate (Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	2-8℃, 1 month
Reference Standard	96T: 2 vials 48T: 1 vial	Discard unused reconstituted standard and dilutions
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8℃
Biotinylated Detection Ab Working Solution	1 vial, 6 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	2-8℃ (Protect from light)
Substrate Reagent	1 vial, 10 mL	2-8℃ (Protect from light)
Stop Solution	1 vial, 10 mL	2-8℃
Plate Sealer	5 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data:

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human TG were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human TG were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	47.23	118.63	485.67	42.51	124.90	446.45
Standard deviation	2.81	5.43	23.94	2.36	5.96	23.97
CV (%)	5.95	4.58	4.93	5.55	4.77	5.37

Recovery

The recovery of Human TG spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	98-110	103
EDTA plasma(n=8)	96-106	103

Target Information

Background

Thyroglobulin (Tg) is a large glycoprotein that is stored in follicles in healthy thyroid tissue as colloid where it acts as a substrate for thyroid hormones synthesis. Thyroglobulin is a homologous dimer with a molecular weight of about 660 kDa. Human protein consists of 2,767 amino acid residues and is the storage form of iodine in the thyroid gland, precursor of the iodinated thyroid hormones, thyroxine (T4) and triiodothyronine (T3). It is exclusively produced by normal or well-differentiated malignant thyrocytes. Then, its tissue-specific origin makes it eminently suitable as a serum tumor indicator in patients followed-up for differentiated thyroid carcinoma (DTC) after thyroid removal and remnants ablation[1].The most studied polymorphisms in the TG gene are rs2069550 (E10SNP158), rs853326 (E12SNP), and rs180223 (E10SNP24) in exons 10–12 and rs2076740 (E33SNP) in exon 33 SNP, which were reported to be associated with AITD[2].
1. Grebe S K G. Diagnosis and management of thyroid carcinoma: a focus on serum thyroglobulin[J]. Expert Review of Endocrinology & Metabolism, 2009, 4(1): 25-43.
2. Wang L Q, Wang T Y, Sun Q L, et al. Correlation between thyroglobulin gene polymorphisms and autoimmune thyroid disease[J]. Molecular medicine reports, 2015, 12(3): 4469-4475.

Database Links	SwissProt: P01266 Entrez Gene: 7038
Synonyms	AITD3, TGN
Research Area	Cancer, Signal transduction

Assay Procedures

	1. Add 50μL standard or sample to the wells, immediately add 50μL Biotinylated Detection Ab working solution to each well. Incubate for 90 min at 37°C
	2. Aspirate and wash the plate for 3 times
	3. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	4. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	5. Add 50μL Stop Solution
	6. Read the plate at 450nm immediately. Calculation of the results