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QuicKey Pro Human ICAM-1/CD54(intercellular adhesion molecule 1) ELISA Kit
SKU: E-OSEL-H0018
QuicKey Pro Human ICAM-1/CD54(intercellular adhesion molecule 1) ELISA Kit
| Detection Range | 0.25-16 ng/mL |
| Sensitivity | 0.15 ng/mL |
Product Details
Properties
| Assay type | Sandwich-ELISA |
| Format | 96T |
| Assay time | 1.5h |
| Detection range | 0.25-16 ng/mL |
| Sensitivity | 0.15 ng/mL |
| Sample type &Sample volume | ; 50μL |
| Specificity | This kit recognizes ICAM-1 in samples. No significant cross-reactivity or interference between ICAM-1 and analogues was observed. |
| Reproducibility | Both intra-CV and inter-CV are < 10%. |
| Application | This ELISA kit applies to the in vitro quantitative determination of ICAM-1 concentrations in . |
Test Principle
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ICAM-1. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human ICAM-1 are added to the micro ELISA plate wells. Human ICAM-1 in samples (or standards) combines with the coated antibody and HRP linked detection antibody specific to ICAM-1. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human ICAM-1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Kit Components and Storage
An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.
| Item | Specifications | Storage |
|---|---|---|
| Micro ELISA Plate(Dismountable) | 96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T |
2-8℃, 1 months |
| Reference Standard | 96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials |
2-8℃, use the reconstituted standard within 24h |
| Concentrated HRP Linked Detection Ab(100×) | 96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL |
2-8℃(Protect from light) |
| Reference Standard & Sample Diluent | 96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL |
2-8℃ |
| HRP Linked Ab Diluent | 96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL |
|
| Concentrated Wash Buffer(25×) | 96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL |
|
| Substrate Reagent | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃(Protect from light) |
| Stop Solution | 96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL |
2-8℃ |
| Plate Sealer | 96T/48T/24T: 5 pieces 96T*5: 25 pieces |
|
| Product Description | 1 copy | |
| Certificate of Analysis | 1 copy |
Technical Data:
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level ICAM-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level ICAM-1 were tested on 3 different plates, 20 replicates in each plate.
| Intra-assay Precision | Inter-assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| Mean (ng/mL) | 0.86 | 2.24 | 5.52 | 0.87 | 2.28 | 5.82 |
| Standard deviation | 0.05 | 0.09 | 0.25 | 0.06 | 0.10 | 0.19 |
| CV (%) | 5.97 | 4.11 | 4.47 | 6.35 | 4.30 | 3.18 |
Recovery
The recovery of ICAM-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
| Sample Type | Range (%) | Average Recovery (%) |
|---|---|---|
| Serum(n=8) | 88-104 | 96 |
| EDTA plasma(n=8) | 87-99 | 94 |
| Cell culture media(n=8) | 91-104 | 96 |
Target Information
| Database Links | SwissProt: P05362 |
| Synonyms | CD54 |
| Research Area | Cancer, Cardiovascular, Immunology, Stem cells |
Assay Procedures
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1. Add 50µL standard or sample to the wells, Immediately add 50µL of HRP linked Detection Ab working solution to each well. Incubate for 60 min at 37°C. |
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2. Aspirate and wash the plate for 5 times. |
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3. Add 90µL of Substrate Reagent. Incubate for about 15 min at 37°C. |
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4. Add 50µL Stop Solution. |
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5. Read the plate at 450nm immediately. Calculation of the results |