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Recombinant Human 14-3-3 epsilon/YWHAE Protein (GST Tag)
SKU: PKSH032016-50
Recombinant Human 14-3-3 epsilon/YWHAE Protein (GST Tag)
| SKU # | PKSH032016 |
| Expression Host | E.coli |
Description
| Synonyms | 14-3-3E, HEL2, KCIP-1, MDCR, MDS |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Gln255 |
| Accession | P62258 |
| Calculated Molecular Weight | 56.0 kDa |
| Observed Molecular Weight | 58 kDa |
| Tag | N-GST |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 6.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
14-3-3 Protein Epsilon acts as an adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. 14-3-3 protein epsilon binds to a large number of partners, usually by recognition of a phophoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner.