Recombinant Human 4-1BBL/TNFSF9 Protein (His Tag)(Active)
SKU: PKSH032023-100
Recombinant Human 4-1BBL/TNFSF9 Protein (His Tag)(Active)
SKU # | PKSH032023 |
Expression Host | E.coli |
Description
Synonyms | 4-1BB ligand, 4-1BBL, TNFSF9, Tumor necrosis factor ligand superfamily member 9 |
Species | Human |
Expression Host | E.coli |
Sequence | Arg71-Asp 140 |
Accession | P41273 |
Calculated Molecular Weight | 20.4 kDa |
Observed Molecular Weight | 20 kDa |
Tag | C-His |
Bio-activity | Measure by its ability to induce IL-8 secretion in human PBMCs. The ED50 for this effect is 1-5 ng/mL. |
Properties
Purity | > 95 % as determined by reducing SDS-PAGE. |
Endotoxin | < 0.1 EU per μg of the protein as determined by the LAL method. |
Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
Formulation | Lyophilized from sterile PBS, pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
Reconstitution | Please refer to the printed manual for detailed information. |
Background
Tumor necrosis factor ligand superfamily member 9(4-1BBL) is single-pass type II membrane protein which is a member of the the tumor necrosis factor family. 4-1BBL is a 254 amino acids cytokine that is expressed in brain; placenta; lung; skeletal muscle and kidney. TNFSF9 has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine may have a role in activation-induced cell death (AICD) and cognate interactions between T-cells and B-cells/macrophages. It has also been shown to be required for the optimal CD8 responses in CD8 T cells; and is thought to be involved in T cell-tumor cell interaction.