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Recombinant Human Activin RIIA/ACVR2A Protein (Fc Tag)(Active)
SKU: PKSH031729-200
Recombinant Human Activin RIIA/ACVR2A Protein (Fc Tag)(Active)
| SKU # | PKSH031729 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | ACTR-IIA, ACTRII, ACTRIIA, ACVR2, ACVR2A, Activin Receptor Type IIA, Activin Receptor Type-2A |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Pro 134 |
| Accession | NP_001607.1 |
| Calculated Molecular Weight | 40.0 kDa |
| Observed Molecular Weight | 60-65 kDa |
| Tag | C-hFc |
| Bio-activity | Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation. The ED50 for this effect is typically 10-40 ng/mL in the presence of 10 ng/mL recombinant Activin A. |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
ACVR2A and ACVR2B are two activin type II receptors. ACVR2A has been shown to interact with INHBA, SYNJ2BP and ACVR1B. The bovine ACVR2A gene encodes a protein of 513 amino acids which is highly homologous (approximately 98% identity) to the rat, mouse, and human ACVR2A proteins. Inactivation of ACVR2A is a common event in prostate cancer cells suggesting it may play an important role in the development of prostate cancer. The ACVR2A gene is a putative tumor suppressor gene that is frequently mutated in microsatellite-unstable colon cancers (MSI-H colon cancers). Frameshift mutation of ACVR2A may contribute to MSI-H colon tumorigenesis via disruption of alternate TGF-beta effector pathways.