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Recombinant Human ALK-2/ACVR1 Protein (His & Fc Tag)(Active)
SKU: PKSH031744-200
Recombinant Human ALK-2/ACVR1 Protein (His & Fc Tag)(Active)
| SKU # | PKSH031744 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | ACTR-I, ACVR1, ACVR1A, ACVRLK2, ALK-2, ALK2, Activin Receptor Type I, Activin Receptor Type-1, Activin Receptor-Like Kinase 2, FOP, SKR1, Serine/Threonine-Protein Kinase Receptor R1, TGF-B Superfamily Receptor Type I, TSR-I |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Val 124 |
| Accession | NP_001104537.1 |
| Calculated Molecular Weight | 39.6 kDa |
| Observed Molecular Weight | 40-45 kDa |
| Tag | C-His-Fc |
| Bio-activity | Measure by its ability to bind with human BMP2 in a functional ELISA. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
ALK-2, also termed as ACVR1, was initially identified as an activin type I receptor because of its ability to bind activin in concert with ActRII or ActRIIB. ALK-2 is also identified as a BMP type I receptor. It has been demonstrated that ALK-2 forms complex with either the BMP-2/7-bound BMPR-II or ACVR2A /ACVR2B. ALK-1 and ALK-2 presenting in the yeast Saccharomyces cerevisiae are two haspin homologues. Both ALK-1 and ALK-2 exhibit a weak auto-kinase activity in vitro, and are phosphoproteins in vivo. ALK-1 and ALK-2 levels peak in mitosis and late-S/G2. Control of protein stability plays a major role in ALK-2 regulation. The half-life of ALK-2 is particularly short in G1. Overexpression of ALK-2, but not of ALK-1, causes a mitotic arrest, which is correlated to the kinase activity of the protein. This suggests a role for ALK-2 in the control of mitosis. Endoglin is phosphorylated on cytosolic domain threonine residues by the TGF-beta type I receptors ALK-2 and ALK-5 in prostate cancer cells. Endoglin did not inhibit cell migration in the presence of constitutively active ALK-2. Defects in ALK-2 are a cause of fibrodysplasia ossificans progressiva (FOP).