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Recombinant Human Astrocytic Phosphoprotein PEA-15/PEA15 Protein
SKU: PKSH032094-50
Recombinant Human Astrocytic Phosphoprotein PEA-15/PEA15 Protein
| SKU # | PKSH032094 |
| Expression Host | E.coli |
Description
| Synonyms | 15 kDa Phosphoprotein Enriched in Astrocytes, Astrocytic Phosphoprotein PEA-15, PEA15, PED, Phosphoprotein Enriched in Diabetes |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Ala130 |
| Accession | Q15121 |
| Calculated Molecular Weight | 15.3 kDa |
| Observed Molecular Weight | 12-16 kDa |
| Tag | None |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Astrocyticphosphoprotein PEA-15 (PEA15) is a death effector domain (DED)-containing protein. PEA15 is mainly expressed in the central nervous system, principally in astrocytes. Increased PEA15 levels affect tumorigenesis and cancer progression. PEA15 is overexpressed in breast cancers and gliomas as well as in type 2 diabetes. PEA15 blocks Ras-mediated inhibition of integrin activation and modulates the ERK MAP kinase cascade. PEA15 also inhibits RPS6KA3 activities by holding it in the cytoplasm. In addition, PEA15 inhibits both TNFRSF6 and TNFRSF1A mediated CASP8 activity and apoptosis. At present, PEA15 expression is also a significant prognostic marker in ovarian cancer.