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Recombinant Human BDH1 Protein (His Tag)
SKU: PKSH032366-50
Recombinant Human BDH1 Protein (His Tag)
| SKU # | PKSH032366 |
| Expression Host | E.coli |
Description
| Synonyms | 3-Hydroxybutyrate Dehydrogenase, BDH, D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Met 1-Arg343 |
| Accession | Q02338 |
| Calculated Molecular Weight | 40.3 kDa |
| Observed Molecular Weight | 40 kDa |
| Tag | N-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 50mM Tris-HCl, 1mM DTT, pH 7.5. |
| Reconstitution | Not Applicable |
Background
D-Beta-Hydroxybutyrate Dehydrogenase Mitochondrial (BDH) is a member of the short-chain dehydrogenases/reductases (SDR) family. BDH is localized in the mitochondrion matrix. BDH forms a homotetrameric lipid-requiring enzyme of the mitochondrial membrane and has a specific necessity for phosphatidylcholine for optimal enzymatic activity. BDH catalyzes the interconversion of acetoacetate and (R)-3-hydroxybutyrate, the 2 main ketone bodies formed during fatty acid catabolism.