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Recombinant Human Carbonic Anhydrase 10/CA10 Protein (E.coli, His Tag)
SKU: PKSH032158-50
Recombinant Human Carbonic Anhydrase 10/CA10 Protein (E.coli, His Tag)
| SKU # | PKSH032158 |
| Expression Host | E.coli |
Description
| Synonyms | CA-RP X, CA-RPX, CA10, CARP X, CARPX, Carbonic Anhydrase-Related Protein 10, Carbonic Anhydrase-Related Protein X, Cerebral Protein 15, HUCEP-15 |
| Species | Human |
| Expression Host | E.coli |
| Sequence | Ala21-Asn300 |
| Accession | Q9NS85 |
| Calculated Molecular Weight | 33.0 kDa |
| Observed Molecular Weight | 31 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 25mM Tris-HCl, 150mM NaCl, pH 7.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Carbonic Anhydrase-Related Protein 10 (CA10) protein belongs to the carbonic anhydrase family of zinc metalloenzymes. It is an acatalytic member of the alpha-carbonic anhydrase subgroup. CA10 expression is detected in the adult total brain and in almost all parts of the central nervous system; but it is not expressed in the fetal brain. CA10 catalyze the reversible hydration of carbon dioxide in various biological processes; which is fundamental to many processes such as respiration; renal tubular acidification and bone resorption. CA10 is thought to play a role in the central nervous system; especially in brain development.