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Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag)
SKU: PKSH032168-50
Recombinant Human Carboxypeptidase A1/CPA1 Protein (His Tag)
| SKU # | PKSH032168 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CPA, CPA1, Carboxypeptidase A1 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Lys17-Tyr419 |
| Accession | AAH05279.1 |
| Calculated Molecular Weight | 46.6 kDa |
| Observed Molecular Weight | 44 kDa |
| Tag | C-His |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles. |
| Shipping | This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C. |
| Formulation | Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 150mm NaCl, pH 7.5. |
| Reconstitution | Not Applicable |
Background
Carboxypeptidase A1 (CPA1) is secreted as a pancreatic peptidase that comes from the precursor form of inactive procarboxypeptidase. CPA1 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. It has a free C-terminal carboxyl group, with the preference of residues with aromatic or branched aliphatic side chains. CPA1 cleaves the C-terminal amide or ester bond of peptides and involves in zymogen inhibition. Three different forms of human pancreatic procarboxypeptidase A have been isolated. In contrast to procarboxypeptidase B which was always secreted by the pancreas as a monomer, procarboxypeptidase A occurs as a monomer and/or associated to one or two functionally different proteins, such as zymogen E.