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Recombinant Human CD31/PECAM1 Protein (Fc Tag)(Active)
SKU: PKSH030454-100
Recombinant Human CD31/PECAM1 Protein (Fc Tag)(Active)
| SKU # | PKSH030454 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CD31, EndoCAM, GPIIA, PECA1, PECAM-1, PECAM1 , Platelet endothelial cell adhesion molecule |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Lys 601 |
| Accession | EAW94208.1 |
| Calculated Molecular Weight | 91.2 kDa |
| Observed Molecular Weight | 130-140 kDa |
| Tag | C-hFc |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When 8 × 104 cells/well are added to sCD31-Fc coated plates (1. 25μg/mL, 100μL/well) in the presence of 20ng/mL PMA, approximately 20-45% will adhere after 30 minutes at 37℃. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile 100mM Glycine, 10mM NaCl, 50mM Tris, pH 7.5 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
The Cluster of Differentiation 31 (CD31) adhesion molecule, also known as platelet-endothelial cell adhesion molecule-1 (PECAM-1), is the only known member of the CAM family on platelets. CD31 protein is a 130-kDa transmembrane glycoprotein expressed by endothelial cells, platelets, monocytes, neutrophils, and certain T cell subsets. CD31 protein is also expressed in certain tumors, including epithelioid hemangioendothelioma, other vascular tumors, and histiocytic malignancies. CD31 plays a key role in removing aged neutrophils and tissue regeneration. CD31 protein mediates the homotypic or heterotypic cell adhesion by binding to itself or the leukocyte integrin αvβ3, and thus plays a role in neutrophil recruitment in inflammatory responses, transendothelial migration of leukocytes, as well as in cardiovascular development.