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Recombinant Human CD40/TNFRSF5 Protein (Fc Tag)(Active)
SKU: PKSH032216-50
Recombinant Human CD40/TNFRSF5 Protein (Fc Tag)(Active)
| SKU # | PKSH032216 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | B-Cell Surface Antigen CD40, Bp50, CD40, CD40L Receptor, CDw40, TNFRSF5, Tumor Necrosis Factor Receptor Superfamily member 5 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Glu21-Arg193 |
| Accession | P25942 |
| Calculated Molecular Weight | 46.3 kDa |
| Observed Molecular Weight | 50-65 kDa |
| Tag | C-Fc |
| Bio-activity | Immobilized Human CD40L-His-Flag at 10μg/ml (100 μl/well)can bind Human CD40-Fc. The ED50 of Human CD40-Fc is 0.45 ug/ml. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS,pH 7.4. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CD40 is a Type I Transmembrane Glycoprotein that belongs to the TNF Receptor Superfamily. CD40 is expressed in B cells; follicular dendritic cells; dendritic cells; activated monocytes; macrophages; endothelial cells; vascular smooth muscle cells; and several tumor cell lines. The extracellular domain of CD40 is characterized by Cysteine rich repeat regions. Interaction of CD40 with its ligand (CD40L) leads to aggregation of CD40 molecules; which in turn interact with cytoplasmic components to initiate signaling pathways. Several different TRAF proteins (adaptor proteins) have been identified to serves as mediators of the signal transduction. CD40 plays an essential role in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching; memory B cell development; and germinal center formation.