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Recombinant Human CD47/IAP Protein (His Tag)(Active)
SKU: PKSH033600-50
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Recombinant Human CD47/IAP Protein (His Tag)(Active)
| SKU # | PKSH033600 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Antigenic Surface Determinant Protein OA3, CD47, IAP, Integrin-Associated Protein, Leukocyte Surface Antigen CD47, MER6, Protein MER6 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gln19-Pro139 |
| Accession | Q08722 |
| Calculated Molecular Weight | 14.8 kDa |
| Observed Molecular Weight | 30-45 kDa |
| Tag | C-His |
| Bio-activity | Loaded Anti-Human CD47 mAb-Fc on Protein A Biosensor, can bind Human CD47-His with an affinity constant of 0.22 nM as determined in BLI assay. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 10mM Tris-Citrate, 150mM NaCl, pH 8.0. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CD47(Integrin-Associated Protein;IAP) is a 40 - 60 kDa variably glycosylated atypical member of the immunoglobulin superfamily. The ubiquitously expressed CD47 binds to SIRP family members on macrophages; neutrophils; and T cells. CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The protein is also a receptor for the C-terminal cell-binding domain of thrombospondin; and it may play a role in membrane transport and signal transduction. This protein has broad tissue distribution; and is reduced in expression on Rh erythrocytes.