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Recombinant Human CD86/B7-2 Protein (aa 1-239,His Tag)(Active)
SKU: PKSH031474-100
Recombinant Human CD86/B7-2 Protein (aa 1-239,His Tag)(Active)
| SKU # | PKSH031474 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Activation B7-2 Antigen, B7-2, B7.2, B70, BU63, CD28LG2, CD86, CTLA-4 Counter-Receptor B7.2, FUN-1, LAB72, T-Lymphocyte Activation Antigen CD86 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-His 239 |
| Accession | NP_008820.2 |
| Calculated Molecular Weight | 26.2 kDa |
| Observed Molecular Weight | 55-60 kDa |
| Tag | C-His |
| Bio-activity | Immobilized human CD86 at 20 μg/ml (100 μl/well) can bind human CD28 with a linear ranger of 32-800 ng/ml. |
Properties
| Purity | > 97 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CD86; also known as B-lymphocyte activation antigen B7-2 (referred to as B70); is a member of the cell surface immunoglobulin superfamily. B7-2 exists predominantly as a monomer on cell surfaces and interacts with two co-stimulatory receptors CD28 and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) expressed on T cells; and thus induces the signal pathways which regulate T cell activation and tolerance; cytokine production; and the generation of CTL. It is indicated that contacts between B and T helper cells mediated by CD86 encourage signals for the proliferation and IgG secretion of normal B cells and B cell lymphomas. Recent study has revealed that CD86 also promotes the generation of a mature APC repertoire and promotes APC function and survival. CD86 has an important role in chronic hemodialysis; allergic pulmonary inflammation; arthritis; and antiviral responses; and thus is regarded as a promising candidate for immune therapy.