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Recombinant Human CD86/B7-2 Protein (Val185Ile, His Tag)(Active)
SKU: PKSH033722-50
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Recombinant Human CD86/B7-2 Protein (Val185Ile, His Tag)(Active)
| SKU # | PKSH033722 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | LAB, ETC, FUN, CTLA-4 Counter-Receptor B, Early T-cell costimulatory molecule, CD28LG, B7-2, B7.2, B70, CD28LG2, LAB72, CD86, Activation B7-2 antigen, BU63, CTLA-4 Counter-Receptor B7.2, Early T-cell costimulatory molecule 1, ETC-1, FUN-1, T-lymphocyte activation antigen CD86, B7-2, Activation B7-2 antigen 3, B7 2, B72 antigen, B-lymphocyte activation antigen B7-2, B-lymphocyte activation antigen B7-2 2, CD28 antigen ligand 2, CD28 antigen ligand 2 2, Cd28l2, CD86 antigen, CD86 molecule, CLS1, CTLA-4 counter-receptor B7.2 2, FUN 1, Ly58, Ly-58, MB7, MB7-2, Membrane glycoprotein, MGC34413, TS/A-2 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ala24-Pro247 |
| Accession | AAH40261.1 |
| Calculated Molecular Weight | 26.7 kDa |
| Observed Molecular Weight | 57-66 kDa |
| Tag | C-His |
| Bio-activity | Loaded Human B7-2-His on HIS1K Biosensor, can bind Mouse CTLA-4-Fc with an affinity constant of 5.65 nM as determined in BLI assay. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.2. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
The protein is the receptor that involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. It may play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T-cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. The protein interacts with MARCH8, human herpesvirus 8 MIR2 protein, adenovirus subgroup B fiber proteins and acts as a receptor for these viruses.It is expressed by activated B-lymphocytes and monocytes and promoted by MARCH8 and results in endocytosis and lysosomal degradation.It contains 1 Ig-like C2-type(immunoglobulin-like) domainand 1 Ig-like V-type (immunoglobulin-like) domain.