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Recombinant Human CHL-1 Protein (His Tag)(Active)
SKU: PKSH031836-50
Recombinant Human CHL-1 Protein (His Tag)(Active)
| SKU # | PKSH031836 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | CALL, L1CAM2, LICAM2 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Met 1-Gln 1080 |
| Accession | AAI04919.1 |
| Calculated Molecular Weight | 120 kDa |
| Observed Molecular Weight | 160-180 kDa |
| Tag | C-His |
| Bio-activity | Measured by the ability of the immobilized protein to support the adhesion of C6 Rat brain glial cells. When 5 x 104 cells/well are added to CHL1 coated plates (0.8 μg/ml and 100 μl/well), approximately 40%-60% will adhere specifically after 60 minutes at 37℃. |
Properties
| Purity | > 95 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
Neural cell adhesion molecule L1-like protein, also known as close homolog of L1 (CHL1) is the prototypic member of the CTF / NF-1 family of transcription factors that serve as a novel calcium signaling pathway-responsive transcription factor and is considered as a member of the largest ctf complementation group, consisting of 30 of 126 ctf mutants isolated. CHL1 is a cell adhesion molecule highly related to L1. It contains structure plan of six extracellular C2-type immunoglobulin (Ig) domains followed by five fibronectin typeⅢ domains linked by a single membrane-spanning region to a short cytoplasmic domain. The extracellular portion of CHL1 is higyly glycosylated and involved them in hemophilic disease.