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Recombinant Human CLEC1B/CLEC2 Protein (His Tag)(Active)
SKU: PKSH031326-100
Recombinant Human CLEC1B/CLEC2 Protein (His Tag)(Active)
| SKU # | PKSH031326 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | 1810061I13Rik, CLEC2, CLEC2B, PRO1384, QDED721 |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Gln 58-Pro 229 |
| Accession | NP_057593.3 |
| Calculated Molecular Weight | 22.7 kDa |
| Observed Molecular Weight | 35-38 kDa |
| Tag | N-His |
| Bio-activity | Immobilized human Podoplanin at 10 μg/mL (100 μl/well) can bind biotinylated human CLEC1B-His, The EC50 of biotinylated human CLEC1B-His is 0.71 μg/mL. |
Properties
| Purity | > 76 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CLEC1B, also known as CLEC2, is a C-type lectin-like receptor expressed in myeloid cells and NK cells. Natural killer (NK) cells express multiple calcium-dependent (C-type) lectin-like receptors, such as CD94 and NKG2D, that interact with major histocompatibility complex class I molecules and either inhibit or activate cytotoxicity and cytokine secretion. CLEC2 acts as a receptor for the platelet-aggregating snake venom protein rhodocytin. Rhodocytin binding leads to tyrosine phosphorylation and this promotes the binding of spleen tyrosine kinase (Syk) and initiation of downstream tyrosine phosphorylation events and activation of PLC-gamma-2. CLEC2 contains 1 C-type lectin domain and is expressed preferentially in the liver. It acts as an attachment factor for human immunodeficiency virus type 1 (HIV-1) and facilitates its capture by platelets.