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Recombinant Human CNDP1 Protein (His Tag)(Active)
SKU: PKSH031891-50
Recombinant Human CNDP1 Protein (His Tag)(Active)
| SKU # | PKSH031891 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Beta-Ala-His Dipeptidase, CN1, CNDP Dipeptidase 1, CNDP1, CPGL2, Carnosine Dipeptidase 1, Glutamate Carboxypeptidase-Like Protein 2, HsT2308, Serum Carnosinase |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Ser27-His507 |
| Accession | NP_116038.4 |
| Calculated Molecular Weight | 55.3 kDa |
| Observed Molecular Weight | 60-65 kDa |
| Tag | C-His |
| Bio-activity | Measured by its ability to cleave carnosine (βAlaLHis) in a twostep assay. The specific activity is > 250 pmoles/min/μg. |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from sterile PBS, pH 7.4 Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
CNDP1; also known as carnosine dipeptidase 1; glutamate carboxypeptidase-like protein 2 (CPGL-2) or carnosinase 1 (CN1); is a member of the M20 metalloprotease family. The CNDP1 gene contains trinucleotide (CTG) repeat length polymorphism in the coding region; which has been demonstrated to be associated with susceptibility to developing diabetic nephropathy; for carnosine protection against the adverse effects of high glucose levels on renal cells. In humans; CNDP1 is secreted from the liver into the serum. In other mammals; including rodents; CNDP1 is expressed exclusively within the kidney and lacks a signal peptide. CNDP1 protein is a secreted homodimeric dipeptidase that specifically hydrolyzes L-carnosine (β-alanyl-L-histidine); and is identified as human carnosinase expressed in the brain. CNDP1 has been associated with diabetic nephropathy in Europeans and European Americans; but not African-Americans. It was identified and confirmed as a risk factor; were cross-sectional and mostly in patients with type 2 diabetes. The polymorphisms of CNDP1 can be excluded as a risk factor for nephropathy in type 1 diabetes. In addition; CNDP1 is also suggested to be implicated in the actions of neuroprotection and neurotransmiting.