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Recombinant Human Coagulation Factor X/F10 Protein (Fc Tag)
SKU: PKSH033714-50
Recombinant Human Coagulation Factor X/F10 Protein (Fc Tag)
| SKU # | PKSH033714 |
| Expression Host | HEK293 Cells |
Description
| Synonyms | Coagulation factor 10, FX, FXA, coagulation factor X |
| Species | Human |
| Expression Host | HEK293 Cells |
| Sequence | Asn32-Lys488 |
| Accession | P00742 |
| Calculated Molecular Weight | 78.2 kDa |
| Observed Molecular Weight | 80-120&25 kDa |
| Tag | C-Fc |
| Bio-activity | Not validated for activity |
Properties
| Purity | > 90 % as determined by reducing SDS-PAGE. |
| Endotoxin | < 1.0 EU per μg of the protein as determined by the LAL method. |
| Storage | Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80℃. Reconstituted protein solution can be stored at 4-8℃ for 2-7 days. Aliquots of reconstituted samples are stable at < -20℃ for 3 months. |
| Shipping | This product is provided as lyophilized powder which is shipped with ice packs. |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM MES, 150mM NaCl, 0.2mM CaCl2, pH 5.5. Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual. |
| Reconstitution | Please refer to the printed manual for detailed information. |
Background
F10, also known as Coagulation factor X, belongs to the peptidase S1 family that is synthesized as a 488 amino acid (aa) with a signal peptide and a pro region (residues 1-40). Both the intrinsic and extrinsic pathways activate Factor X to Xa, which consists of light (residues 41-179) and heavy (residues 235-488) chains linked by a disulfide bond. Coagulation factor X is initially synthesized in the liver. The two chains are formed from a single-chain precursor by the excision of two Arg residues and are held together by 1 or more disulfide bonds. Forms a heterodimer with SERPINA5. F10 is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting.